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Use of an Injection Port for Thermochemolysis-Gas chromatography/mass Spectrometry: Rapid Profiling of Biomaterials

Use of an Injection Port for Thermochemolysis-Gas chromatography/mass Spectrometry: Rapid Profiling of Biomaterials

Farzad Shadkami, Robert Helleur

J Chromatogr A. 2009 Jul 31;1216(31):5903-10.

PMID: 19559444

Abstract:

A simple and direct approach was developed for thermochemolytic analysis of a wide range of biomolecules present in plant materials using an injection port of a gas chromatograph/mass spectrometer (GC/MS) and a novel solids injector consisting of a coiled stainless steel wire placed inside a modified needle syringe. Optimum thermochemolysis (or Thermally Assisted Hydrolysis/Methylation) was achieved by using a suitable methanolic solution of trimethylsulfonium hydroxide (TMSH) or tetramethylammonium hydroxide (TMAH) with an injection port temperature of 350 degrees C. Intact, methylated flavonoids, saccharides, phenolic and fatty acids, lignin dimers and diterpene resin acids were identified. Samples include tea leaves, hemicelluloses, lignin isolates and herbal medicines. Unexpected chromatographic results using TMAH reagent revealed the presence of intact methylated trisaccharides (658 Da) and structurally informative dimer lignin markers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP17287035	Trimethylsulfonium hydroxide solution		17287-03-5	Price

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