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Use of Autoclave Extraction and Liquid Chromatography With Tandem Mass Spectrometry for Determination of Maleic Hydrazide Residues in Tobacco

Use of Autoclave Extraction and Liquid Chromatography With Tandem Mass Spectrometry for Determination of Maleic Hydrazide Residues in Tobacco

Huimin Deng, Zhaoyang Bian, Fei Yang, Shanshan Liu, Zhonghao Li, Ziyan Fan, Ying Wang, Gangling Tang

J Sep Sci. 2019 Jul;42(14):2390-2397.

PMID: 31038270

Abstract:

Maleic hydrazide has been extensively used as an effective growth regulator in tobacco sucker control. After application, maleic hydrazide distributes itself throughout the tobacco plant where it can exist as free, or forms glucoside conjugates with glucose, or becomes bound with lignin. Among them, free maleic hydrazide and its glucoside conjugates are extractable under conventional solvent extraction, while lignin bound maleic hydrazide is claimed to be non-extractable. Herein, an autoclave extraction method has been developed to extract maleic hydrazide effectively, in which tobacco samples are extracted in an autoclave at 130°C for 1 h using 4 M hydrochloric acid. Under such pressurized hot acidic water conditions, lignin bound maleic hydrazide can be released. Meanwhile, glucoside conjugates are hydrolyzed. Total maleic hydrazide is detected by liquid chromatography coupled with tandem mass spectrometry, and the quantitative results coincide well with that obtained from the international standard method. The proposed autoclave extraction with liquid chromatography and tandem mass spectrometry method exhibits excellent linearity in the range of 5-200 mg/kg (R2 = 0.9998), the matrix matched limit of detection and limit of quantification is 0.68 and 2.27 mg/kg, respectively. This method is simple and improves sample capacity, providing an effective approach to monitoring maleic hydrazide residues in tobacco.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
CS353694	Maleic hydrazide-13C4			Price

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