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Use of CK-548 and CK-869 as Arp2/3 Complex Inhibitors Directly Suppresses Microtubule Assembly Both in Vitro and in Vivo

Use of CK-548 and CK-869 as Arp2/3 Complex Inhibitors Directly Suppresses Microtubule Assembly Both in Vitro and in Vivo

Yuka Yamagishi, Kazumasa Oya, Akira Matsuura, Hiroshi Abe

Biochem Biophys Res Commun. 2018 Feb 12;496(3):834-839.

PMID: 29395083

Abstract:

Two types of Arp2/3 complex inhibitors, CK-666/636 and CK-548/869, are commonly used to study Arp2/3 complex-dependent actin assembly both in vitro and in vivo. However, we found that CK-548 and CK-869 directly suppress microtubule (MT) assembly independent of the actin cytoskeleton. Treatment of cultured mammalian cells with 50 μM CK-869 dramatically decreased MT networks and, instead, accumulated tubulin at the cell periphery, as did nocodazole that inhibits MT assembly. An in vitro MT-sedimentation assay revealed that CK-548 and CK-869 significantly suppressed MT polymerization. In budding yeast, although CK-548 and CK-869 are reported to lack binding abilities in the yeast Arp3, CK-548 treatment decreased cytoplasmic MT at several tens of micromolar concentrations. In addition, we found that the effects of CK-548 and CK-869 on MT assembly varied according to species. We propose that CK-548 and CK-869 are not suitable for studying the cytoskeleton in living cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP388592447	CK-869		388592-44-7	Price

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