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Utility of Eosin Y as a Complexing Reagent for the Determination of Citalopram Hydrobromide in Commercial Dosage Forms by Fluorescence Spectrophotometry

Utility of Eosin Y as a Complexing Reagent for the Determination of Citalopram Hydrobromide in Commercial Dosage Forms by Fluorescence Spectrophotometry

Syed Najmul Hejaz Azmi, Ahlam Al-Fazari, Munira Al-Badaei, Ruqiya Al-Mahrazi

Luminescence. 2015 Dec;30(8):1352-9.

PMID: 25829234

Abstract:

An accurate, selective and sensitive spectrofluorimetric method was developed for the determination of citalopram hydrobromide in commercial dosage forms. The method was based on the formation of a fluorescent ion-pair complex between citalopram hydrobromide and eosin Y in the presence of a disodium hydrogen phosphate/citric acid buffer solution of pH 3.4 that was extractable in dichloromethane. The extracted complex showed fluorescence intensity at λem = 554 nm after excitation at 259 nm. The calibration curve was linear over at concentrations of 2.0-26.0 µg/mL. Under optimized experimental conditions, the proposed method was validated as per ICH guidelines. The effect of common excipients used as additives was tested and the tolerance limit calculated. The limit of detection for the proposed method was 0.121 μg/mL. The proposed method was successfully applied to the determination of citalopram hydrobromide in commercial dosage forms. The results were compared with the reference RP-HPLC method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP59729327	Citalopram hydrobromide solution		59729-32-7	Price

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