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[Validation of a Liquid Chromatography Method for Rifampicin Determination in Human Plasma]

[Validation of a Liquid Chromatography Method for Rifampicin Determination in Human Plasma]

Luis Moreno-Exebio, Miguel Grande-Ortiz

Rev Peru Med Exp Salud Publica. 2014;31(1):56-61.

PMID: 24718527

Abstract:

Objectives:
To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma.
Materials and methods:
A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used.
Results:
The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods.
Conclusions:
The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7778770-A	Monobasic potassium phosphate		7778-77-0	Price

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