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Validation of Pyrithiobac Sodium (Staple Herbicide) ELISA for Australian Cotton Soils

Validation of Pyrithiobac Sodium (Staple Herbicide) ELISA for Australian Cotton Soils

Shuo Wang, Helen L Beasley, Sheldon R Sumpter, Ivan R Kennedy

J Environ Sci Health B. 2003 May;38(3):281-91.

PMID: 12716046

Abstract:

An enzyme-linked immunosorbent assay (ELISA) for pyrithiobac-sodium (Staple) produced by DuPont was validated in Australian soils. This pyrithiobac-sodium ELISA was shown to be highly sensitive with the limit of detection of 4-5 ppt. Soil samples were extracted either in PBS buffer by shaking or by accelerated solvent extraction (ASE). While pyrithiobac sodium can be analyzed directly by ELISA after ASE extraction with 1/10 or more dilutions, the analysis of PBS extract required filtration and dilution 1/20 or more depending on the concentration. Immunoassay results compared favorably with GC-MS results for both ASE and PBS extract of incurred residue of pyrithiobac sodium in soil samples, indicating that this ELISA can be an inexpensive and reliable alternative to conventional residue analysis methods for quantification of pyrithiobac-sodium. This validation provided the basis for applying the ELISA to a field study of pyrithiobac-sodium.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP123342938	Pyrithiobac		123342-93-8	Price

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