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Vancomycin-loaded Oxidized Hyaluronic Acid and Adipic Acid Dihydrazide Hydrogel: Bio-compatibility, Drug Release, Antimicrobial Activity, and Biofilm Model

Vancomycin-loaded Oxidized Hyaluronic Acid and Adipic Acid Dihydrazide Hydrogel: Bio-compatibility, Drug Release, Antimicrobial Activity, and Biofilm Model

Chun-Hsing Liao, Chiang Sang Chen, Yu-Chun Chen, Ni-En Jiang, Chui Jia Farn, Yi-Shan Shen, Ming-Lun Hsu, Chih-Hung Chang

J Microbiol Immunol Infect. 2019 Sep 30;S1684-1182(19)30141-0.

PMID: 31607570

Abstract:

Background:
Prosthesis infection is a difficult-to-treat situation. Hydrogel is a novel biomaterial, which can be applied by simply spraying or by coating on implants before surgery and can be easily mixed with antibiotics.
Methods:
In order to evaluate the potential use of antibiotic-loaded hydrogel, we incorporated vancomycin into oxidized hyaluronic acid (HA) and adipic acid dihydrazide and evaluated the drug release and antimicrobial activity against methicillin-resistant Staphylococcus aureus (ATCC 29213).
Results:
The average release percentage of vancomycin on day 3 was about 86%. The antibiotic-loaded gel was biocompatible with mesenchymal stem cell, MC3T3, and L929 cell lines. The in vitro inhibition zones of vancomycin-loaded hydrogel [500X minimal inhibition concentration (MIC), 50X MIC, 10X MIC, and blank hydrogel] were 21, 13, 9, and 5 mm, respectively. In the Ti6Al4V implant biofilm model, 0.01-1% vancomycin-loaded gel exhibited significant anti-biofilm activity, measured by the MTT assay.
Conclusions:
Vancomycin could be loaded onto oxidized HA and adipic acid dihydrazide, which exhibited excellent drug release and in vitro antimicrobial activity with minimal cell toxicity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1071938	Adipic acid dihydrazide		1071-93-8	Price

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