Home
Resources
Publications
Verapamil and Norverapamil Determination in Human Plasma by Gas-Liquid Chromatography Using Nitrogen-Phosphorus Detection: Application to Single-Dose Pharmacokinetic Studies

Verapamil and Norverapamil Determination in Human Plasma by Gas-Liquid Chromatography Using Nitrogen-Phosphorus Detection: Application to Single-Dose Pharmacokinetic Studies

D R Abernethy, E L Todd, J R Mitchell

Pharmacology. 1984;29(5):264-8.

PMID: 6494236

Abstract:

A sensitive (to 0.5 ng/ml) and specific method for the determination of verapamil and norverapamil which utilizes gas-liquid chromatography with nitrogen-phosphorus detection is described. A basic extraction with acid back-wash and final basic reextraction is used for the preparation of plasma samples. Standard curves using alpha-isopropyl-alpha-[(N-methyl-N-homoveratryl)-beta-aminoethyl]- 3,4- dimethoxyphenylacetonitrile hydrochloride (D-517) are linear for concentrations from 0.5 to 200 ng/ml for both verapamil and norverapamil. Within-day and between-day reproducibility is good with a coefficient of variation less than 10% for all concentrations. Recovery is complete for both verapamil and norverapamil. Application of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg verapamil hydrochloride by intravenous infusion.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP67812424	(±)-Norverapamil hydrochloride		67812-42-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: