Home
Resources
Publications
Voltammetric Investigation of Macrolides by an HPLC-coulometric Assay

Voltammetric Investigation of Macrolides by an HPLC-coulometric Assay

Yong-Hak Kim, Jairaj V Pothuluri, Carl E Cerniglia

J Pharm Biomed Anal. 2005 Jul 1;38(3):390-6.

PMID: 15925238

Abstract:

Voltammograms of macrolides, including anhydroerythromycin A, azithromycin, erythromycin A, erythromycin A enol ether, pseudoerythromycin A enol ether, oleandomycin and tylosin have been investigated using a dual electrode cell in combination with a high-throughput LC method. The half-wave potentials (E(1/2)) of the seven macrolides investigated ranged from 0.734 to 0.866 V, and the current responses reached the maxima at over 1.0 V. The current response of the downstream electrode displayed a non-linear behavior at high potentials over +0.75 V, probably because of polarization of solvent components, e.g., water. The HPLC-coulometric assay was optimized with the potentials of the upstream and downstream electrodes at +0.65 and +0.85 V, respectively. This method is suitable for detection of 14- and 15-membered macrolides (sensitivity<0.05 microg ml(-1)), but not for a 16-membered macrolide, tylosin (sensitivity>0.1 microg ml(-1)). The assay shows interferences from biomatrices in rat's blood plasma and serum, and human urine, but they were effectively removed by a cold acetonitrile extraction method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP105882697	Pseudoerythromycin A enol ether		105882-69-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: