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Voltammetric Sensing of Phosphoproteins Using a gallium(III) Acetylacetonate-Modified Carbon Paste Electrode

Voltammetric Sensing of Phosphoproteins Using a gallium(III) Acetylacetonate-Modified Carbon Paste Electrode

Kazuharu Sugawara, Asako Yugami, Toshihiko Kadoya

Anal Sci. 2012;28(3):251-5.

PMID: 22451365

Abstract:

The voltammetric detection of phosphoproteins was developed using a gallium(III) acetylacetonate-modified carbon paste electrode. Because phosphate groups of the protein interacted with the gallium(III) ion, the protein was accumulated on the electrode surface. A hexaammine ruthenium(III) ion, which combined with the functional groups, was used to monitor the interaction. When phosvitin and hexaammine ruthenium(III) ions were incubated in 0.1 M acetate buffer (pH 3.2), a reduction peak of hexaammine ruthenium(III) ion at the electrode decreased as the concentration of the protein increased. In contrast, an increase in the peak current was observed with a plain carbon paste electrode. These results were caused by a competitive reaction of the phosphate groups with the hexaammine ruthenium(III) and gallium(III) ions. In the presence of α-, β- and κ-caseins, the electrode response decreased due to the order of the numbers of phosphate groups. This method could be applied to the sensing of phosphoproteins at the 10(-10) M level.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14284936	Ruthenium(III) acetylacetonate		14284-93-6	Price

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