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Wnt-1 Signaling Inhibits Human Umbilical Vein Endothelial Cell Proliferation and Alters Cell Morphology

Wnt-1 Signaling Inhibits Human Umbilical Vein Endothelial Cell Proliferation and Alters Cell Morphology

Ching-wen Cheng, Stephen K Smith, D Stephen Charnock-Jones

Exp Cell Res. 2003 Dec 10;291(2):415-25.

PMID: 14644163

Abstract:

Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. There are many factors which can mediate this interaction including Wnt-signaling-related molecules. Wnt signaling is involved in many developmental processes and cellular functions. There is increasing evidence suggesting that Wnt signaling has a role in regulating endothelial cell growth although the precise mechanism is unclear. In this study, we established a coculture system to examine how Wnt-1 signaling regulates human umbilical vein endothelial cell (HUVEC) growth and behavior. We found that Wnt-1 signals inhibited BrdU incorporation in HUVECs and the number of labeled cells also decreased in proportion to the number of Wnt-1-expressing cells present (P < 0.05). Moreover, HUVECs cocultured with Wnt-1-expressing C57MG cells clumped together rather than remaining scattered throughout the culture. These effects were dependent on cell contact. Treatment of HUVEC with LiCl, which inhibits the activity of GSK-3beta and mimicked Wnt-1 signaling, also inhibited the BrdU incorporation in endothelial cells. Our results suggest that Wnt signaling has a role in endothelial cell growth control and this is mediated through cell-cell contact. They also suggest that Wnt signaling might participate in angiogenesis by regulating endothelial cell growth and function.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248785	WNT-1 human			Price

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