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Xylanase Immobilization on Magnetite and Magnetite core/shell Nanocomposites Using Two Different Flexible Alkyl Length Organophosphonates: Linker Length and Shell Effect on Enzyme Catalytic Activity

Xylanase Immobilization on Magnetite and Magnetite core/shell Nanocomposites Using Two Different Flexible Alkyl Length Organophosphonates: Linker Length and Shell Effect on Enzyme Catalytic Activity

Vishal Singh, Sunaina Kaul, Prinka Singla, Vinod Kumar, Rajat Sandhir, Jong Hoon Chung, Pankaj Garg, Nitin Kumar Singhal

Int J Biol Macromol. 2018 Aug;115:590-599.

PMID: 29684449

Abstract:

Magnetite and magnetite core/shell (Fe3O4/SiO2) nanoparticles were synthesized and functionalized with two different alkyl chain length linkers that were 3-Phosphonopropionic acid (3-PPA) and 16-Phosphonohexadecanoic acid (16-PHDA). Xylanase (EC 3.2.1.8, endo-1,4-xylanase, endo-1), was immobilized on as synthesized bare and silica coated magnetite nanoparticles via well-known EDC coupling. Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM), X-Ray Diffraction Spectroscopy (XRD), X-ray Photoelectron Spectroscopy (XPS), Dynamic Light Scattering (DLS) and Thermogravimetric analysis (TGA) techniques were utilized to characterize all the modifications. The flexible linker chain length plays a vital role in the catalytic attributes of the immobilized enzyme. Result shows that long chain alkyl linker grafted magnetite and magnetite core/silica shell nanoparticles exhibited a superior performance in terms of lower Km, higher catalytic efficiency and better reusability. Furthermore, the immobilized xylanase shows improved tolerability performance at a wide range of pH and temperature. Silica-coated magnetite nanoparticles bound xylanase through 16-PHDA retained 90% of its initial activity after 10 consecutive cycles, further emphasize on the beneficial effect of linker chain length and inert silica coating.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP443361180	16-Phosphonohexadecanoic acid		443361-18-0	Price

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