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Yarrowia Lipolytica Lipase Lip2: An Efficient Enzyme for the Production of Concentrates of Docosahexaenoic Acid Ethyl Ester

Yarrowia Lipolytica Lipase Lip2: An Efficient Enzyme for the Production of Concentrates of Docosahexaenoic Acid Ethyl Ester

Leticia Casas-Godoy, Muchalin Meunchan, Marlène Cot, Sophie Duquesne, Florence Bordes, Alain Marty

J Biotechnol. 2014 Jun 20;180:30-6.

PMID: 24657346

Abstract:

The production of Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) rich in cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) was studied using lipase-catalysed hydrolysis of a mixture of ethyl esters from tuna oil. Lipases from Yarrowia lipolytica (YLL2), Thermomyces lanuginosus (TLL) and Candida rugosa (CRL1, CRL3 and CRL4) were tested. C. rugosa lipases discriminated esters on the basis of their chain length, with less affinity for γ-linolenate, 11-eicosenoate, arachidonate, EPA, DPA and DHA ethyl esters. However, YLL2 and TLL improved discrimination towards DHA, as enzyme selectivity was shown to be mainly based on the position of the double bond closest to the carboxylic group. From the point of view of kinetics, purity and yield, YLL2 was the most effective lipase for DHA purification. Using this enzyme in an open reactor process resulted in the highest concentrations of DHA ethyl ester (77%) and ω-3 esters (81%) with a recovery of 94% and 77% respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP84494724-B	cis-4,7,10,13,16,19-Docosahexaenoic acid ethyl ester		84494-72-4	Price

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