CAS 12240-15-2 Berlin Blue - Analytical Products / Alfa Chemistry

CAT	APB12240152
CAS	12240-15-2
Synonyms	Potassium iron(III) hexacyanoferrate (ii); Prussian blue soluble
Molecular Weight	306.89 (anhydrous basis)
Molecular Formula	C6Fe2KN6·xH2O

Solubility	Soluble in water
Appearance	Blue powder
Color Index	77520
Grade	For microscopy / Spazial
Uses	Used for tracking of transplanted cells such as mesenchymal stem cells. Used to detect iron in tissue sections. Used to differentiate slow versus fast cycling cells by methods that monitor iron oxide particle labeling dilution thru cell division.

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Case Study

Prussian Blue Used for the Preparation of NIR-Responsive CO-Releasing Hydrogel for Infected Wound Therapy

Li, Yao, et al. Chemical Engineering Journal 512 (2025): 162544.

In this study, Prussian Blue (PB) was used as a key precursor in the fabrication of a nanocomposite hydrogel for infected wound treatment via carbon monoxide (CO) gas therapy. The preparation began with the synthesis of hollow Prussian Blue nanoparticles (HPB NPs). Specifically, 5 g of PVP was dissolved in 0.01 M HCl, followed by the addition of 0.6 g of K₃[Fe(CN)₆]. After 20 h of heating at 80 °C, PB NPs were formed. These were further treated with 1 M HCl and PVP, then hydrothermally processed at 140 °C to yield HPB NPs.
To load CO donors, 2 mg of HPB NPs, 10 mg of Fe₃(CO)₁₂, and 4 mg of 1-pentadecanol were sonicated in ethanol, followed by 24 h stirring under N₂ atmosphere in the dark. The resulting HPB-CO NPs were collected by centrifugation.
For hydrogel fabrication, oxidized sodium alginate (OSA) was synthesized via periodate oxidation of sodium alginate. A solution of HPB-CO NPs was blended with Q-CMC and OSA to form HPB-CO@gel. To introduce bioactivity, PDRN was dissolved into OSA before mixing.
This NIR-responsive hydrogel exhibited controlled CO release and enhanced healing properties, demonstrating the functional value of Prussian Blue in developing responsive biomaterials for clinical wound therapy.

Prussian Blue Used for the Modification of Screen-Printed Electrodes for Electrochemical Detection of Amyloid-β Peptides

Suprun, Elena V., et al. Electrochimica Acta 406 (2022): 139829.

Prussian Blue (PB) was effectively utilized for the modification of carbon screen-printed electrodes (SPE), enabling enhanced electrochemical detection of amyloid-β (Aβ) peptides through flow injection amperometry. The PB-modified electrodes (ΓPB = 1.9-2.5 nmol·cm⁻²) demonstrated excellent redox performance in phosphate buffer (pH 6.0, 0.1 M KCl), showing two well-defined redox couples: PB/Prussian White (PW) and Berlin Green (BG)/PB, with formal potentials of 119 mV and 817 mV, respectively.
The modified SPEs showed remarkable catalytic enhancement (up to 27-fold increase in current) for Aβ peptide variants lacking the Tyr-10 residue, such as Aβ(1-7)-D7H and rat Aβ(1-16). Cyclic voltammetry confirmed the high reversibility and conductivity of the PB film, evidenced by small peak separations (ΔEp as low as 8 mV) and peak current ratios (Ip,a/Ip,c ≈ 1) over a sweep rate range of 2-200 mV·s⁻¹.
The log-log plots of peak current versus sweep rate yielded slopes near 1, characteristic of adsorption-controlled processes, indicating strong and stable anchoring of PB on the electrode surface. This system enables sensitive detection of peptides that lack inherently electroactive residues, suggesting a valuable tool for electrochemical biosensing of modified or truncated peptides, with potential applications in Alzheimer's research.
This study highlights the utility of Prussian Blue in developing high-performance electrochemical sensors for challenging biomolecular targets.

Prussian Blue Used for the Preparation of FeCx@C Catalysts for Fischer-Tropsch Synthesis

Li, Bingshuang, et al. Applied Catalysis A: General 638 (2022): 118609.

Prussian Blue (Fe₄[Fe(CN)₆]₃) was employed as a single-source precursor for the synthesis of Fe carbide species encapsulated in a carbon matrix (FeCx@C), serving as highly tunable catalysts for Fischer-Tropsch Synthesis (FTS). By thermally treating PB under an inert argon atmosphere, the FeCx phase and carbon shell structure were precisely controlled via calcination temperature.
The synthesis involved a two-stage heating protocol: PB was first heated to 200 °C at 2 °C/min and held for 3 h, followed by calcination at 450-600 °C for 4 h to yield a series of catalysts, designated as PB-C-450 to PB-C-600. As temperature increased, the FeCx core phase evolved, and the carbon shell thickened. Structural analyses revealed a temperature-dependent decarburization process proceeding from the particle interior to the exterior.
Catalytic performance in FTS demonstrated that higher calcination temperatures enhanced the carbon-chain growth probability (α value), correlating with optimized carbon encapsulation and FeCx phase formation. This suggests that PB-derived FeCx@C catalysts can be finely engineered for selective hydrocarbon production by modulating the thermal treatment conditions.
This study showcases the versatility of Prussian Blue as a molecular precursor to produce structurally controlled Fe-based catalysts, offering a straightforward route to tailor catalytic activity and selectivity in Fischer-Tropsch applications.