Obeticholic Acid-D5

INQUIRY Add to cart

For Research Use Only | Not For Clinical Use

CAT	APB1992000802
CAS	1992000-80-2
Molecular Weight	425.66
Molecular Formula	C26H39D5O4

* Products or Services Interested: Obeticholic Acid-D5 (1992000-80-2)
* Your question
* Name
* Email
Verification Code

Case Study

Internal Standards of Obeticholic Acid-D5 (OBT d5) for Accurate Quantification in LC-MS/MS Analysis

Valluri, Venkat Rao, et al. Journal of Separation Science 44.7 (2021): 1307-1323.

The use of internal standards (ISTDs) is critical for achieving high precision and accuracy in liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. Obeticholic Acid-D5 (OBT d5), along with its glycine and taurine conjugates, serves as an essential ISTD for the quantification of obeticholic acid (OBT) and its pharmacologically active metabolites in human plasma.
In a validated LC-MS/MS method, OBT d5 demonstrated superior performance in compensating for matrix effects, instrumental fluctuations, and sample preparation variability. The study utilized ISTDs OBT d5 (99.52%), glyco-OBT d5 (98.80%), and tauro-OBT d5 sodium salt (99.32%), ensuring accurate quantification of OBT and its metabolites. The chromatographic separation was performed using a phenyl column with a mobile phase of 0.05% acetic acid and HPLC-grade methanol (10:90, v/v) at a 0.4 mL/min flow rate.
The mass spectrometer, operated in negative electrospray ionization mode, detected OBT at m/z 419.2 and its ISTD OBT d5 at m/z 424.1. The method demonstrated excellent linearity and was successfully applied in pharmacokinetic studies. These findings highlight the indispensable role of OBT d5 as an ISTD in bioanalytical applications, ensuring reliable and reproducible quantification of obeticholic acid and its metabolites in clinical research.

Obeticholic Acid-D5 as an Internal Standard for the Quantification of Obeticholic Acid and Its Metabolites in Human Plasma Using UPLC-MS/MS

Li, Sha, et al. Journal of Chromatography B 1246 (2024): 124296.

Obeticholic acid-d5 (OCA-d5) is a stable isotope-labeled internal standard (ISTD) used for the accurate quantification of obeticholic acid (OCA) and its metabolites in bioanalytical applications. In a validated ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, OCA-d5 demonstrated exceptional performance in ensuring precise measurement of OCA and its major metabolites, glyco-obeticholic acid (GOA) and tauro-obeticholic acid (TOA), in human plasma.
The study employed solid-phase extraction (SPE) followed by UPLC-MS/MS analysis, utilizing OCA-d5 as an ISTD to correct for variability in extraction efficiency and instrument response. The mass-to-charge transition (m/z) for OCA-d5 was monitored at 424.4 → 424.4 in multiple reaction monitoring (MRM) mode, ensuring high specificity. The method achieved a quantitative range of 0.2506-100.2 ng/mL for OCA with excellent accuracy and precision. Extraction recoveries of OCA-d5 were determined to be 87.18%, with matrix effects normalized to an acceptable range (CV < 15%).
The validated method was successfully applied to pharmacokinetic studies, demonstrating the critical role of OCA-d5 in ensuring reliable bioanalysis of OCA. Its high recovery and stability make it an indispensable ISTD for quantitative drug analysis in clinical and pharmaceutical research.

Send Us a Request

What is your specific need? We will do everything we can to meet your expectations.

Online Inquiry