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A Role for Phosphoinositides in Tyrosine Phosphorylation of p125 Focal Adhesion Kinase in Rat Pancreatic Acini

A Role for Phosphoinositides in Tyrosine Phosphorylation of p125 Focal Adhesion Kinase in Rat Pancreatic Acini

J A Rosado, G M Salido, L J García

Cell Signal. 2000 Mar;12(3):173-82.

PMID: 10704824

Abstract:

Previous studies have shown that different agonists increase tyrosine phosphorylation of the focal adhesion related proteins p125(FAK), p130(Cas), and paxillin in different cell types and that tyrosine phosphorylation depends on the integrity of the actin cytoskeleton. Because phosphoinositides are important for the maintenance of the cytoskeleton, the role of phosphoinositides in the tyrosine phosphorylation of these proteins in response to occupancy of m3 muscarinic and CCK(A) receptors has been investigated in pancreatic acini. Addition of carbachol or CCK-8 to pancreatic acini resulted in rapid increases in the tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin. Pretreatment of pancreatic acini with LY294002 or wortmannin resulted in a concentration-dependent inhibition of tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin stimulated by carbachol or CCK-8. Carbachol- or CCK-8-stimulated tyrosine phosphorylation of these proteins was not inhibited by rapamycin, PD 98059 or SB 203580, and thus it was dissociated from the activation of p70 S6 or MAP kinases. These results indicate that m3 muscarinic and CCK(A) receptor-mediated increase in p125(FAK), p130(Cas), and paxillin tyrosine phosphorylation in pancreatic acini depends on the ability of these cells to synthesise phosphoinositides.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP152121476-B	SB 203580 - CAS 152121-47-6	152121-47-6	Price
AP167869218-C	PD 98059 - CAS 167869-21-8	167869-21-8	Price
AP19545267-B	Wortmannin - CAS 19545-26-7	19545-26-7	Price

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