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Activation of Wiskott-Aldrich Syndrome Protein and Its Association With Other Proteins by Stromal Cell-Derived factor-1alpha Is Associated With Cell Migration in a T-lymphocyte Line

Activation of Wiskott-Aldrich Syndrome Protein and Its Association With Other Proteins by Stromal Cell-Derived factor-1alpha Is Associated With Cell Migration in a T-lymphocyte Line

Seiichi Okabe, Seiji Fukuda, Hal E Broxmeyer

Exp Hematol. 2002 Jul;30(7):761-6.

PMID: 12135674

Abstract:

Objectives:
Chemokines play a central role in lymphocyte trafficking and homing. The actin cytoskeleton is involved in cell morphological changes and motility. Wiskott-Aldrich syndrome (WAS) protein (WASP) has been implicated in regulation of cytoskeleton rearrangement. To evaluate mechanisms that might be involved in migration of T cells, we examined effects of stromal cell-derived factor (SDF)-1alpha on WASP and associated proteins.
Methods:
Jurkat T cells were stimulated by SDF-1alpha and analyzed for chemotaxis and also by Western blot analysis for signal transduction.
Results:
Jurkat T cells displayed chemotaxis to SDF-1alpha, which was inhibited by pretreatment of cells with either pertussis toxin, a Galphai protein inhibitor, wortmannin or Ly294002, phophatidylinositol 3-kinase inhibitors, or herbimycin, a protein tyrosine kinase inhibitor. WASP was tyrosine phosphorylated in response to SDF-1alpha stimulation in Jurkat T cells. Crk associated substrate (Cas), Nck, and focal adhesion kinase (FAK) were also phosphorylated after SDF-1alpha stimulation. Moreover, activated Nck interacted with Cas and WASP as determined by co-immunoprecipitation, and FAK also bound to Cas.
Conclusions:
These data suggest that WASP, Cas, Nck, and FAK may play a role in SDF-1alpha-induced migration of the T-cell line, Jurkat.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19545267-B	Wortmannin - CAS 19545-26-7		19545-26-7	Price

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