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Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells

Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells

Xiaofen Ni, Jiajun Chen, Fengying Lu, Zhengzhong Yuan, Xiaofeng Xu, Zhen Hu, Lei Jiang

Recent Pat Anticancer Drug Discov. 2018;13(2):240-247.

PMID: 29600769

Abstract:

Background:
α-Solanine, the most important and active component of Solanum nigrum, was found to have anti-cancer activity on multiple cancer cells. However, its effects on colorectal cancer (CRC) and associated molecular mechanisms remain to be further elucidated.
Objective:
In this study, we investigated the anti-cancer effects of α-solanine against CRC cells in vitro and in vivo.
Materials & methods:
Cell viability was measured using Cell Counting Kit-8 (CCK-8) assay; cell cycle was analyzed with a Cycletest Plus DNA Reagent Kit; cell apoptosis was detected by flow cytometer; cell migration and invasive ability was determined by Transwell assays; S100P protein expression was also analyzed by western blotting; lentiviral vectors expressing shRNA targeting the S100P gene.
Results:
We demonstrated that α-solanine inhibited CRC cell (SW480, SW620 and HT-29) growth as well as migration and invasion, induced cell cycle arrest and apoptosis in vitro, and suppressed tumor growth in vivo. Moreover, we observed that S100P expression was downregulated by α-solanine. Overexpression of S100P partially reversed the α-solanine-induced growth inhibition of CRC cells. Conversely, knockdown of S100P by lentiviral-mediated RNAi resulted in significantly promoting the α-solanine-induced growth inhibition.
Conclusion:
These findings suggest that α-solanine is a potential agent for the treatment of CRC, and the anti-tumor effect of α-solanine in the CRC cells may be mediated at least partly by the downregulation of S100P.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20562021	α-Solanine		20562-02-1	Price

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