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Calphostin C Induces Expression of Amphiregulin mRNA via Reactive Oxygen Species in IEC-6 Cells

Calphostin C Induces Expression of Amphiregulin mRNA via Reactive Oxygen Species in IEC-6 Cells

Y Miyazaki, Y Shinomura, S Tsutsui, S Kitamura, S Hiraoka, Y Matsuzawa

Life Sci. 1998;63(25):PL361-5.

PMID: 9870716

Abstract:

Calphostin C, a secondary metabolite of the fungus Cladosporium cladosporioides, is generally used as a specific inhibitor of protein kinase C. It is known that 12-O-tetradecanoyl-13-phorbol acetate (TPA), a protein kinase C activator, induces expression of mRNA for amphiregulin (AR), a member of EGF-related polypeptides, in mammalian epithelial cells. In this work, we determined the effect of calphostin C on AR mRNA expression in IEC-6 cells, a rat intestinal epithelial cell line, and unexpectedly found that this compound enhanced the TPA-induced expression of AR mRNA. Moreover, calphostin C alone induced expression of AR mRNA in a light-dependent manner, and this effect was abrogated by pretreatment with N-acetylcysteine. These results suggest that calphostin C can upregulate expression of AR mRNA via reactive oxygen species.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP121263192-A	Calphostin C from Cladosporium cladosporioides		121263-19-2	Price

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