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Characterization of Chromanol 293B-induced Block of the Delayed-Rectifier K+ Current in Heart-Derived H9c2 Cells

Characterization of Chromanol 293B-induced Block of the Delayed-Rectifier K+ Current in Heart-Derived H9c2 Cells

Yi-Ching Lo, Su-Rong Yang, Mei-Han Huang, Yen-Chin Liu, Sheng-Nan Wu

Life Sci. 2005 Apr 1;76(20):2275-86.

PMID: 15748622

Abstract:

The effects of chromanol 293B on ion currents in rat embryonic heart-derived H9c2 cells were investigated in this study. Chromanol 293B suppressed the amplitude of delayed rectified K+ current (I(K)) in a concentration-dependent manner. The IC50 value for chromanol 293B-induced inhibition of I(K) was 8 microM. The I(K) present in these cells, the electrical properties of which resembled those for the Kv2.1-related K+ current, was sensitive to inhibition by quinidine or dendrotoxin, yet not by pandinotoxin-Kalpha, E-4031 or apamin. Chromanol 293B reduced the activation time constant of I(K) and the effective gating charge of this channel. However, little or no modification in the steady-state inactivation of I(K) in response to long-lasting conditioning pulses could be demonstrated in the presence of chromanol 293B. These results clearly demonstrate that chromanol 293B can effectively interact with the K+ channel functionally expressed in H9c2 myoblasts. The chromanol 293B-induced inhibition of these channels could primarily be attributed to open channel block.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP163163233	Chromanol 293B		163163-23-3	Price
AP185529640	Pandinotoxin-Kα		185529-64-0	Price

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