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Covalent and Allosteric Inhibitors of the ATPase VCP/p97 Induce Cancer Cell Death

Covalent and Allosteric Inhibitors of the ATPase VCP/p97 Induce Cancer Cell Death

Paola Magnaghi, Roberto D'Alessio, Barbara Valsasina, Nilla Avanzi, Simona Rizzi, Daniela Asa, Fabio Gasparri, Liviana Cozzi, Ulisse Cucchi, Christian Orrenius, Paolo Polucci, Dario Ballinari, Claudia Perrera, etc.

Nat Chem Biol. 2013 Sep;9(9):548-56.

PMID: 23892893

Abstract:

VCP (also known as p97 or Cdc48p in yeast) is an AAA(+) ATPase regulating endoplasmic reticulum-associated degradation. After high-throughput screening, we developed compounds that inhibit VCP via different mechanisms, including covalent modification of an active site cysteine and a new allosteric mechanism. Using photoaffinity labeling, structural analysis and mutagenesis, we mapped the binding site of allosteric inhibitors to a region spanning the D1 and D2 domains of adjacent protomers encompassing elements important for nucleotide-state sensing and ATP hydrolysis. These compounds induced an increased affinity for nucleotides. Interference with nucleotide turnover in individual subunits and distortion of interprotomer communication cooperated to impair VCP enzymatic activity. Chemical expansion of this allosteric class identified NMS-873, the most potent and specific VCP inhibitor described to date, which activated the unfolded protein response, interfered with autophagy and induced cancer cell death. The consistent pattern of cancer cell killing by covalent and allosteric inhibitors provided critical validation of VCP as a cancer target.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1418013758	NMS-873		1418013-75-8	Price

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