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Determination of Acetaldehyde in Fuel Ethanol by High-Performance Liquid Chromatography With Electrochemical Detection

Determination of Acetaldehyde in Fuel Ethanol by High-Performance Liquid Chromatography With Electrochemical Detection

Adelir A Saczk, Leonardo L Okumura, Marcelo F De Oliveira, Maria Valnice B Zanoni, Nelson R Stradiotto

Anal Sci. 2005 Apr;21(4):441-4.

PMID: 15844343

Abstract:

The cyclic voltammetric behavior of acetaldehyde and the derivatized product with 2,4-dinitrophenylhydrazine (DNPHi) has been studied at a glassy carbon electrode. This study was used to optimize the best experimental conditions for its determination by high-performance liquid chromatographic (HPLC) separation coupled with electrochemical detection. The acetaldehyde-2,4-dinitrophenylhydrazone (ADNPH) was eluted and separated by a reversed-phase column, C18, under isocratic conditions with the mobile phase containing a binary mixture of methanol/LiCl(aq) at a concentration of 1.0 x 10(-3) M (80:20 v/v) and a flow rate of 1.0 mL min(-1). The optimum condition for the electrochemical detection of ADNPH was +1.0 V vs. Ag/AgCl as a reference electrode. The proposed method was simple, rapid (analysis time 7 min) and sensitive (detection limit 3.80 microg L(-1)) at a signal-to-noise ratio of 3:1. It was also highly selective and reproducible [standard deviation 8.2% +/- 0.36 (n = 5)]. The analytical curve of ADNPH was linear over the range of 3-300 mg L(-1) per injection (20 microL), and the analytical recovery was > 99%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1019574	Acetaldehyde-2,4-dinitrophenylhydrazone		1019-57-4	Price

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