Acetaldehyde-2,4-dinitrophenylhydrazone

Name: Acetaldehyde-2,4-dinitrophenylhydrazone
SKU: AP1019574
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For Research Use Only | Not For Clinical Use

Catalog NumberAP1019574

CAS Number1019-57-4

CategoryAnalytical Standards

Case Study

Acetaldehyde-2,4-dinitrophenylhydrazone Used for the Quantitative Analysis of Aldehyde Emissions in E-Cigarette Aerosols

El Mubarak, Mohamed A., et al. Food and Chemical Toxicology 116 (2018): 147-151.

Acetaldehyde-2,4-dinitrophenylhydrazone is employed as a key analytical derivative in the quantification of aldehydes produced from the thermal decomposition of e-liquid base components such as vegetable glycerin and propylene glycol. Upon heating in electronic cigarette atomizers, these substances generate harmful aldehydes including formaldehyde, acetaldehyde, acrolein, and crotonaldehyde. To enable accurate detection and quantification, the volatile aldehydes are captured via derivatization with 2,4-dinitrophenylhydrazine (DNPH) and subsequently analyzed as stable hydrazone derivatives.
Using high-performance liquid chromatography (HPLC) on a Jupiter Proteo 90A column with a gradient elution of 0.1% formic acid in water and acetonitrile at 0.4 mL/min, the method enables simultaneous quantitation of multiple DNPH-derivatized aldehydes. Acetaldehyde-2,4-dinitrophenylhydrazone demonstrates excellent chromatographic behavior, contributing to reliable linearity (R² ≥ 0.999), high sensitivity (LOD: 0.008 μg/mL; LOQ: 0.025 μg/mL), and acceptable intra-/inter-day accuracy and precision (≤10% RSD and RE).
Calibrators and quality control samples prepared in acetonitrile further validated the robustness of the method. Among tested base liquids, vegetable glycerin was identified as the highest emitter of aldehydes. This validated protocol highlights the essential role of acetaldehyde-2,4-dinitrophenylhydrazone in aldehyde emission profiling and offers a valuable tool for future assessments of e-cigarette safety and regulatory control.

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MDL Number	MFCD00458721
Molecular Weight	224.17
InChI Key	ONBOQRNOMHHDFB-XNWCZRBMSA-N

Description	certified Reference Material
Form	neat
Grade	certified Reference Material
Linear Formula	CH3CH=NNHC6H3(NO2)2
MP	165-168 °C (lit.)
Size	10MG
Storage Conditions	2-8°C