Acetaldehyde-2,4-dinitrophenylhydrazone

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For Research Use Only | Not For Clinical Use

CAT	AP1019574
CAS	1019-57-4
MDL Number	MFCD00458721
Molecular Weight	224.17
InChI Key	ONBOQRNOMHHDFB-XNWCZRBMSA-N

Description	certified Reference Material
Form	neat
Grade	certified Reference Material
Linear Formula	CH3CH=NNHC6H3(NO2)2
MP	165-168 °C (lit.)
Size	10MG
Storage Conditions	2-8°C

2-(exo-tricyclo[5.2.1.0(2,6)]deca-4,8-dien-3-endo-yl)acetaldehyde 2,4-dinitrophenylhydrazone

A Abdul Ajees, N Palani, K K Balasubramanian

Acta Crystallogr C. 2002 Jul;58(Pt 7):o433-5.

PMID: 12094069

Determination of Acetaldehyde in Fuel Ethanol by High-Performance Liquid Chromatography With Electrochemical Detection

Adelir A Saczk, Leonardo L Okumura, Marcelo F De Oliveira, Maria Valnice B Zanoni, Nelson R Stradiotto

Anal Sci. 2005 Apr;21(4):441-4.

PMID: 15844343

Development of an Automated Measurement System Using a Diffusion Scrubber and High-Performance Liquid Chromatography for the Monitoring of Formaldehyde and Acetaldehyde in Automotive Exhaust Gas

Y Komazaki, Y Narita, S Tanaka

Analyst. 1998 Nov;123(11):2343-9.

PMID: 10396812

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Case Study

Acetaldehyde-2,4-dinitrophenylhydrazone Used for the Quantitative Analysis of Aldehyde Emissions in E-Cigarette Aerosols

El Mubarak, Mohamed A., et al. Food and Chemical Toxicology 116 (2018): 147-151.

Acetaldehyde-2,4-dinitrophenylhydrazone is employed as a key analytical derivative in the quantification of aldehydes produced from the thermal decomposition of e-liquid base components such as vegetable glycerin and propylene glycol. Upon heating in electronic cigarette atomizers, these substances generate harmful aldehydes including formaldehyde, acetaldehyde, acrolein, and crotonaldehyde. To enable accurate detection and quantification, the volatile aldehydes are captured via derivatization with 2,4-dinitrophenylhydrazine (DNPH) and subsequently analyzed as stable hydrazone derivatives.
Using high-performance liquid chromatography (HPLC) on a Jupiter Proteo 90A column with a gradient elution of 0.1% formic acid in water and acetonitrile at 0.4 mL/min, the method enables simultaneous quantitation of multiple DNPH-derivatized aldehydes. Acetaldehyde-2,4-dinitrophenylhydrazone demonstrates excellent chromatographic behavior, contributing to reliable linearity (R² ≥ 0.999), high sensitivity (LOD: 0.008 μg/mL; LOQ: 0.025 μg/mL), and acceptable intra-/inter-day accuracy and precision (≤10% RSD and RE).
Calibrators and quality control samples prepared in acetonitrile further validated the robustness of the method. Among tested base liquids, vegetable glycerin was identified as the highest emitter of aldehydes. This validated protocol highlights the essential role of acetaldehyde-2,4-dinitrophenylhydrazone in aldehyde emission profiling and offers a valuable tool for future assessments of e-cigarette safety and regulatory control.

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