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Effect of Snuff and Nicotine on DNA Methylation by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone

Effect of Snuff and Nicotine on DNA Methylation by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone

G Prokopczyk, J D Adams, E J LaVoie, D Hoffmann

Carcinogenesis. 1987 Oct;8(10):1395-7.

PMID: 3652377

Abstract:

In this study we assayed the effects of snuff and nicotine on the DNA methylation by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a powerful tobacco-specific N-nitrosamine. Male F344 rats were pretreated for 2 weeks with either a solution of a snuff extract or 0.002% nicotine in the drinking water. Subsequently, the rats were given a single dose of NNK and the effects of snuff and nicotine on the methylation of guanine by NNK in the DNA of target organs of this carcinogenic nitrosamine were determined. Formation of 7-methylguanine in the liver, nasal mucosae and oral cavity and of O6-methylguanine in the liver and oral cavity was much lower in the rats pretreated with snuff extract than in those not pretreated. On the other hand, pretreatment of the rats with nicotine had no significant effect on the methylation of DNA by NNK nor on the elimination constants of NNK and its major metabolite 4-(methylnitrosamino)-1-(3-pyridly)-1-butanol.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64091914-A	4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) solution		64091-91-4	Price

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