Home
Resources
Publications
Esculetin (6,7-dihydroxycoumarin): A Potential Cancer Chemopreventive Agent Through Suppression of Sp1 in Oral Squamous Cancer Cells

Esculetin (6,7-dihydroxycoumarin): A Potential Cancer Chemopreventive Agent Through Suppression of Sp1 in Oral Squamous Cancer Cells

Jin Hyoung Cho, Jae-Cheon Shin, Jung-Jae Cho, Yung Hyun Choi, Jung-Hyun Shim, Jung-Il Chae

Int J Oncol. 2015 Jan;46(1):265-71.

PMID: 25310400

Abstract:

Esculetin (6,7-dihydroxycoumarin), a coumarin compound, is known to inhibit proliferation and induce apoptosis in several types of human cancer cells and is regarded as a promising chemotherapeutic agent. The purpose of the present study was to investigate the anti-proliferative effects of esculetin on two oral squamous cell carcinoma (OSCC) cell lines, HN22 and HSC4, through regulation of specificity protein 1 (Sp1). We examined the apoptotic effects of esculetin were measured by MTS assay, DAPI staining, Annexin V, PI staining, RT-PCR, western blot analysis and immunocytochemistry in HN22 and HSC4 cells. Taken together, the results of the present study indicate that esculetin had anti-proliferative effect on the growth of OSCC cells (HN22 and HSC4) in a dose- and time-dependent manner. The treatment of HN22 and HSC4 cells with esculetin led to a significant reduction in growth and induced apoptosis, followed by the regulation of Sp1 and Sp1 regulatory protein. This indicates that esculetin inhibited cell growth and induced apoptosis by suppressing Sp1 in HN22 and HSC4 cells, suggesting it to be a potent anticancer drug candidate for oral cancer.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP305011	6,7-Dihydroxycoumarin		305-01-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: