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Ginsenoside Rb2 Suppresses the Glutamate-Mediated Oxidative Stress and Neuronal Cell Death in HT22 Cells

Ginsenoside Rb2 Suppresses the Glutamate-Mediated Oxidative Stress and Neuronal Cell Death in HT22 Cells

Dong Hoi Kim, Dae Won Kim, Bo Hyun Jung, Jong Hun Lee, Heesu Lee, Gwi Seo Hwang, Ki Sung Kang, Jae Wook Lee

J Ginseng Res. 2019 Apr;43(2):326-334.

PMID: 30976171

Abstract:

Background:
The objective of our study was to analyze the neuroprotective effects of ginsenoside derivatives Rb1, Rb2, Rc, Rd, Rg1, and Rg3 against glutamate-mediated neurotoxicity in HT22 hippocampal mouse neuron cells.
Methods:
The neuroprotective effect of ginsenosides were evaluated by measuring cell viability. Protein expressions of mitogen-activated protein kinase (MAPK), Bcl2, Bax, and apoptosis-inducing factor (AIF) were determined by Western blot analysis. The occurrence of apoptotic and death cells was determined by flow cytometry. Cellular level of Ca2+ and reactive oxygen species (ROS) levels were evaluated by image analysis using the fluorescent probes Fluor-3 and 2',7'-dichlorodihydrofluorescein diacetate, respectively. In vivo efficacy of neuroprotection was evaluated using the Mongolian gerbil of ischemic brain injury model.
Result:
Reduction of cell viability by glutamate (5 mM) was significantly suppressed by treatment with ginsenoside Rb2. Phosphorylation of MAPKs, Bax, and nuclear AIF was gradually increased by treatment with 5 mM of glutamate and decreased by co-treatment with Rb2. The occurrence of apoptotic cells was decreased by treatment with Rb2 (25.7 μM). Cellular Ca2+ and ROS levels were decreased in the presence of Rb2, and in vivo data indicated that Rb2 treatment (10 mg/kg) significantly diminished the number of degenerated neurons.
Conclusion:
Our results suggest that Rb2 possesses neuroprotective properties that suppress glutamate-induced neurotoxicity. The molecular mechanism of Rb2 is by suppressing the MAPKs activity and AIF translocation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP11021139-A	Ginsenoside Rb2		11021-13-9	Price

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