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High Performance Liquid Chromatography With Ultraviolet Detection for the Determination of SYUIQ-5, a Novel Telomerase Inhibitor for Cancer Therapy: Application to an Enzyme Kinetic Study in Rat Liver Microsomes

High Performance Liquid Chromatography With Ultraviolet Detection for the Determination of SYUIQ-5, a Novel Telomerase Inhibitor for Cancer Therapy: Application to an Enzyme Kinetic Study in Rat Liver Microsomes

Qi-Biao Su, Fan He, Su Guan, Yu-Jing Lu, Lian-Quan Gu, Zhi-Shu Huang, Xiao Chen, Min Huang, Chun-Guang Li, Balram Chowbay, Shu-Feng Zhou

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):332-7.

PMID: 17449337

Abstract:

A sensitive assay for the determination of SYUIQ-5, a novel telomerase inhibitor and anti-tumor drug, in rat liver microsomes was developed by using high-performance liquid chromatography with ultraviolet detection. SYUIQ-5 was incubated in vitro with liver microsomes from rats pre-treated with control vehicle, beta-naphthofIavone, phenobarbital, 20% ethanol or dexamethasone. The analytes were extracted with diethyl ether and separated a C(18) 5-microm analytical column. Elution was conducted with 30 mM dipotassium hydrogen phosphate (pH 8.0)-methanol-triethylamine (30:70:0.05, v/v/v) at a flow-rate of 1.0 ml/min and the detection of UV absorbance was conducted at 278 nm. Intra-day and inter-day precision and accuracy of the method were within 10%. The mean analytical recoveries of SYUIQ-5 ranged from 78.8 to 95.3%. The linearity of the calibration curve was in the range of 1.0-80.0 microM. The lower limit of quantification (LOQ) was 1.0 microM. Kinetic analysis showed that beta-naphthofIavone and dexamethasone significantly induced SYUIQ-5 metabolism, suggesting that cytochrome P450 1A and 3A are the major contributor to SYUIQ-5 metabolism in rat liver microsomes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP188630479	SYUIQ-5		188630-47-9	Price

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