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ICRF-193 Modifies Etoposide-Induced Apoptosis in Thymocytes

ICRF-193 Modifies Etoposide-Induced Apoptosis in Thymocytes

C Tanimoto, S Hirakawa, H Kawasaki, N Hayakawa, Z Ota

Acta Med Okayama. 1995 Dec;49(6):281-6.

PMID: 8770236

Abstract:

Etoposide (VP-16), one of the topoisomerase II (TopoII) inhibitors, interferes with TopoII by inducing the formation of and stabilizing a cleavable enzyme-DNA complex. VP-16 has been demonstrated to induce apoptosis in murine thymocytes. To clarify the mechanism of action of VP-16, we examined the in vitro effect of a non-cleavable-complex-forming type TopoII inhibitor, ICRF-193 which inhibits the DNA strand breakage induced by VP-16, on murine thymocytes in which apoptosis had been induced with VP-16. DNA fragmentation is characteristic of apoptosis. In the early stages, ICRF-193 decreased DNA fragmentation induced by VP-16, although this inhibitory effect decreased in the later. These data suggest that TopoII inhibitors induce apoptosis in murine thymocytes in two ways: with DNA-strand breaks in the early stage or without them. ICRF-193 itself induced apoptosis in murine thymocytes. The time course of DNA fragmentation caused by ICRF-193 was different from that of VP-16.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP21416886	ICRF-193		21416-88-6	Price

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