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In Vitro Reconstruction of Tetronate RK-682 Biosynthesis

Yuhui Sun, Frank Hahn, Yuliya Demydchuk, James Chettle, Manuela Tosin, Hiroyuki Osada, Peter F Leadlay

Nat Chem Biol. 2010 Feb;6(2):99-101.

PMID: 20081823

Abstract:

The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein-bound substrates.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP150627375 RK-682 RK-682 150627-37-5 Price
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