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Loss of [3H]4-DAMP Binding to Muscarinic Receptors in the Orbitofrontal Cortex of Alzheimer's Disease Patients With Psychosis

Loss of [3H]4-DAMP Binding to Muscarinic Receptors in the Orbitofrontal Cortex of Alzheimer's Disease Patients With Psychosis

S W Y Tsang, P T Francis, M M Esiri, P T H Wong, C P L H Chen, M K P Lai

Psychopharmacology (Berl). 2008 Jun;198(2):251-9.

PMID: 18373228

Abstract:

Rationale:
Neuropsychiatric behaviours in Alzheimer's disease (AD) patients have been associated with neocortical alterations of presynaptic cholinergic and muscarinic M2 receptor markers. In contrast, it is unclear whether non-M2 muscarinic receptors have a role to play in AD behavioural symptoms.
Objectives:
To correlate the alterations of neocortical postsynaptic muscarinic receptors with clinical features of AD.
Materials and methods:
[(3)H]4-DAMP were used in binding assays with lysates of Chinese hamster ovary (CHO) cells stably transfected with M1-M5 receptors. [(3)H]4-DAMP was further used to measure muscarinic receptors in the postmortem orbitofrontal cortex of aged controls and AD patients longitudinally assessed for cognitive decline and behavioural symptoms.
Results:
[(3)H]4-DAMP binds to human postmortem brain homogenates and M1-, M3-, M4- and M5-transfected CHO lysates with subnanomolar affinity. Compared to the controls, the [(3)H]4-DAMP binding density is reduced only in AD patients with significant psychotic symptoms. The association between reduced [(3)H]4-DAMP binding and psychosis is independent of the effects of dementia severity or neurofibrillary tangle burden.
Conclusions:
This study suggests that the loss of non-M2 muscarinic receptors in the orbitofrontal cortex may be a neurochemical substrate of psychosis in AD and provides a rationale for further development of muscarinic receptor ligands in AD pharmacotherapy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1952154	4-DAMP		1952-15-4	Price

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