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Luminol, Horseradish Peroxidase, and Glucose Oxidase Ternary Functionalized Graphene Oxide for Ultrasensitive Glucose Sensing

Luminol, Horseradish Peroxidase, and Glucose Oxidase Ternary Functionalized Graphene Oxide for Ultrasensitive Glucose Sensing

Fang Li, Wenjing Ma, Jiachang Liu, Xiang Wu, Yan Wang, Jianbo He

Anal Bioanal Chem. 2018 Jan;410(2):543-552.

PMID: 29167935

Abstract:

Luminol, horseradish peroxidase (HRP), and glucose oxidase (GOx) ternary functionalized graphene oxide (HRP/GOx-luminol-GO) with excellent chemiluminescence (CL) activity and specific enzymatic property was prepared via a simple and general strategy for the first time. In this approach, luminol functionalized GO (luminol-GO) was prepared by gently stirring GO with luminol. Then HRP and GOx were further co-immobilized onto the surface of luminol-GO by storing HRP and GOx with luminol-GO at 4 °C overnight, to form HRP/GOx-luminol-GO bionanocomposites. The synthesized HRP/GOx-luminol-GO could react with H2O2 generated from GOx catalyzed glucose oxidization reaction, to produce strong CL emission in the presence of co-immobilized HRP. Thus, we developed an ultrasensitive, homogeneous, reagentless, selective, and simple CL sensing system for glucose detection. The resulting biosensors exhibited ultra-wide linear range from 5.0 nM to 5.0 mM, and an ultra-low detection limit of 1.2 nM, which was more than 3 orders of magnitude lower than previously reported methods. Furthermore, the sensing system was successfully applied for the detection of glucose in human blood samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP521313	Luminol		521-31-3	Price

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