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Molecular Cloning and Expression in Streptomyces Lividans of a Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

Molecular Cloning and Expression in Streptomyces Lividans of a Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

F Malpartida, M Zalacaín, A Jiménez, J Davies

Biochem Biophys Res Commun. 1983 Nov 30;117(1):6-12.

PMID: 6318753

Abstract:

The gene encoding the phosphotransferase enzyme that modifies hygromycin B in its producing organism Streptomyces hygroscopicus, has been cloned in the Streptomyces vector pIJ41. Two plasmids, pFM4 and pFM6, containing 2.1 and 19.6 kb inserts of Streptomyces hygroscopicus DNA, respectively, which express the modifying enzyme, have been isolated. A 3.1 kb PstI restriction fragment from pFM4 was inserted in the Streptomyces vector pIJ350 and the resulting plasmids, pMZ11.1 and pMZ11.2, express the hygromycin B-resistance phenotype. The utility of this dominant marker for cloning experiments is discussed in the text.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP31282049	Hygromycin B from Streptomyces hygroscopicus		31282-04-9	Price

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