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Neutrophil Elastase Enhances IL-12p40 Production by Lipopolysaccharide-Stimulated Macrophages via Transactivation of the PAR-2/EGFR/TLR4 Signaling Pathway

Neutrophil Elastase Enhances IL-12p40 Production by Lipopolysaccharide-Stimulated Macrophages via Transactivation of the PAR-2/EGFR/TLR4 Signaling Pathway

Rui Yamaguchi, Takatoshi Yamamoto, Arisa Sakamoto, Shinji Narahara, Hiroyuki Sugiuchi, Yasuo Yamaguchi

Blood Cells Mol Dis. 2016 Jul;59:1-7.

PMID: 27282560

Abstract:

Proteinase-activated receptor 2 (PAR-2) and toll-like receptor 4 (TLR4) are involved in innate immune responses and signaling cross-talk between these receptor molecules has the potential to augment an ongoing inflammatory response. The aim of this study was to evaluate the possible cooperative influence of PAR-2 and TLR4 on IL-12p40 production by macrophages after stimulation with lipopolysaccharide (LPS). During culture, GM-CSF upregulated PAR-2 expression by macrophages in a time-dependent manner. Stimulation with LPS enhanced IL-12p40 production by macrophages in a concentration-dependent manner. While human neutrophil elastase (HNE) did not induce IL-12p40 production, pretreatment of macrophages with HNE synergistically increased the IL-12p40 protein level after LPS exposure. Silencing of TLR4 with small interfering RNA blunted the synergistic enhancement of IL-12p40 by HNE combined with LPS. Silencing of β-arrestin 2, p22phox, or ERK1/2 also inhibited an increase of IL-12p40. Interestingly, transfection of macrophages with small interfering RNA duplexes for DUOX-2, EGFR, TLR4, or TRAF6 significantly blunted the increase of IL-12p40 in response to treatment with HNE plus LPS. U73122 and Rottlerin also inhibited the increased production of IL-12p40. In conclusion, HNE is involved in transactivation of TLR4 through activation of DUOX-2/EGFR and synergistically enhances IL-12p40 production by macrophages stimulated with LPS.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248651	IL-12p40 human			Price

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