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Nucleotide Sequence of the Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

Nucleotide Sequence of the Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

M Zalacain, A González, M C Guerrero, R J Mattaliano, F Malpartida, A Jiménez

Nucleic Acids Res. 1986 Feb 25;14(4):1565-81.

PMID: 3005976

Abstract:

The nucleotide sequence of a 1467 bp fragment of Streptomyces hygroscopicus DNA containing the gene (hyg) encoding a hygromycin B phosphotransferase (HPH) has been determined. The N-terminal amino acid sequence of HPH determined by automated Edman degradation has allowed the coding sequence of the hyg gene to be identified. The translation initiation triplet is GTG and 5 bp preceding it there is a sequence complementary to the 3'-end of 16S rRNA from S. lividans. The transcriptional start and termination sites have been determined; the presumptive promoter region has only partial homology to that of the Streptomyces vinaceus vph gene and is different to the promoter sequences of other Streptomyces genes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP31282049	Hygromycin B from Streptomyces hygroscopicus		31282-04-9	Price

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