0

Proteome-wide Drug and Metabolite Interaction Mapping by Thermal-Stability Profiling

Kilian V M Huber, Karin M Olek, André C Müller, Chris Soon Heng Tan, Keiryn L Bennett, Jacques Colinge, Giulio Superti-Furga

Nat Methods. 2015 Nov;12(11):1055-7.

PMID: 26389571

Abstract:

Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs methotrexate and (S)-crizotinib and the metabolite 2'3'-cGAMP in intact cells and identified the 2'3'-cGAMP cognate transmembrane receptor STING, involved in immune signaling.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1374356452 (S)-Crizotinib (S)-Crizotinib 1374356-45-2 Price
qrcode