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Rev-erbα Regulates Circadian Rhythms and StAR Expression in Rat Granulosa Cells as Identified by the Agonist GSK4112

Rev-erbα Regulates Circadian Rhythms and StAR Expression in Rat Granulosa Cells as Identified by the Agonist GSK4112

Huatao Chen, Guiyan Chu, Lijia Zhao, Nobuhiko Yamauchi, Yasufumi Shigeyoshi, Seiichi Hashimoto, Masa-aki Hattori

Biochem Biophys Res Commun. 2012 Apr 6;420(2):374-9.

PMID: 22425774

Abstract:

The Rev-erbα gene is regarded as a circadian clock gene and clock-regulated gene which regulates the circadian transcriptional/translational loop in a subtle way. Here, we first detected the circadian oscillation in mature granulosa cells from antral follicles using a real-time monitoring system of Per2 promoter activity with the addition of FSH. Then we used GSK4112, an agonist ligand of Rev-erbα, to investigate the function of Rev-erbα. GSK4112 treatment significantly reduced the Per2-dLuc amplitude and induced the Per2 oscillation phase advance shift. GSK4112 significantly inhibited Bmal1 mRNA expression, whereas it did clearly stimulate expression of StAR mRNA in a dose-dependent manner. Our data are the first to show the Rev-erbα function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erbα may coordinate circadian rhythm and metabolism in rat ovaries.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4249366	REV-ERBα Agonist, GSK4112			Price

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