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RO-3306 Prevents Postovulatory Aging-Mediated Spontaneous Exit From M-II Arrest in Rat Eggs Cultured in Vitro

RO-3306 Prevents Postovulatory Aging-Mediated Spontaneous Exit From M-II Arrest in Rat Eggs Cultured in Vitro

Shilpa Prasad, Biplob Koch, Shail K Chaube

Biomed Pharmacother. 2016 Mar;78:216-225.

PMID: 26898445

Abstract:

Background:
Postovulatory aging-mediated spontaneous exit from metaphase-II (M-II) arrest deteriorates egg quality and limits assisted reproductive technologies outcome (ART) outcome. Present study was aimed to find out whether RO-3306, specific cyclin dependent kinase 1 (Cdk1) inhibitor could protect against postovulatory aging-mediated spontaneous exit from M-II arrest in rat eggs cultured in vitro.
Methods:
Freshly ovulated M-II arrested eggs were exposed to various concentrations of RO-3306 for 3h in vitro. The morphological changes, percentage of spontaneous exit from M-II arrest, total and specific phosphorylation status of Cdk1, cyclin B1 level and Cdk1 activity were analyzed.
Results:
Data suggest that RO-3306 protected postovulatory aging-mediated spontaneous exit from M-II arrest in a concentration-dependent manner. Postovulatory aging increased Thr14/Tyr15 phosphorylated Cdk1 level, decreased Thr161 phosphorylated Cdk1 as well as cyclin B1 levels and increased Cdk1 activity in aged eggs cultured in vitro. On the other hand, RO-3306 protected postovulatory aging-induced changes in specific phosphorylation of Cdk1, cyclin B1 level, inhibited the kinase activity and prevented spontaneous exit from M-II arrest.
Conclusions:
Our results suggest that postovulatory aging destabilizes MPF by modulating specific phosphorylation of Cdk1 and cyclin B1 level. RO-3306 prevented these changes and maintained M-II arrest in rat eggs cultured in vitro. Hence, maintenance of M-II arrest in ovulated eggs using RO-3306 could be beneficial to increase the number of eggs available for various ART programs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP872573938	RO-3306		872573-93-8	Price

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