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Simplified Methods for Isolation of Ubiquitin From Erythrocytes. Generation of Ubiquitin Polymers

Simplified Methods for Isolation of Ubiquitin From Erythrocytes. Generation of Ubiquitin Polymers

H F Deutsch

Int J Biochem. 1987;19(11):1055-61.

PMID: 2828133

Abstract:

1. Ubiquitin has been isolated from bovine erythrocytes by procedures in which the hemoglobin was removed by denaturation with either ethanol-chloroform mixtures or by heating. 2. The proteins soluble to the denaturation step were removed by 3% sodium trichloroacetate (TCA) at pH 2.0-2.5 or by 5% TCA. 3. Ubiquitin was isolated in relatively high yield from the TCA insoluble fraction by use of single ion-exchange chromatographic and gel permeation steps. 4. Ubiquitin shows relatively little cross-linking upon treatment with glutaraldehyde or with dimethyl suberimidate. Heating of the glutaraldehyde treated material in 4 M guanidine, however, leads to marked aggregation. 5. The polymers of ubiquitin react strongly with antibody in an immunoblot assay.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP79586224	Ubiquitin from bovine erythrocytes		79586-22-4	Price

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