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Structural Basis of Cooling Agent and Lipid Sensing by the Cold-Activated TRPM8 Channel

Structural Basis of Cooling Agent and Lipid Sensing by the Cold-Activated TRPM8 Channel

Ying Yin, Son C Le, Allen L Hsu, Mario J Borgnia, Huanghe Yang, Seok-Yong Lee

Science. 2019 Mar 1;363(6430):eaav9334.

PMID: 30733385

Abstract:

Transient receptor potential melastatin member 8 (TRPM8) is a calcium ion (Ca2+)-permeable cation channel that serves as the primary cold and menthol sensor in humans. Activation of TRPM8 by cooling compounds relies on allosteric actions of agonist and membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), but lack of structural information has thus far precluded a mechanistic understanding of ligand and lipid sensing by TRPM8. Using cryo-electron microscopy, we determined the structures of TRPM8 in complex with the synthetic cooling compound icilin, PIP2, and Ca2+, as well as in complex with the menthol analog WS-12 and PIP2 Our structures reveal the binding sites for cooling agonists and PIP2 in TRPM8. Notably, PIP2 binds to TRPM8 in two different modes, which illustrate the mechanism of allosteric coupling between PIP2 and agonists. This study provides a platform for understanding the molecular mechanism of TRPM8 activation by cooling agents.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4243636	Icilin			Price
IAR4245588	WS-12			Price

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