Home
Resources
Publications
The AAA ATPase Vps4 Plays Important Roles in Candida Albicans Hyphal Formation and Is Inhibited by DBeQ

The AAA ATPase Vps4 Plays Important Roles in Candida Albicans Hyphal Formation and Is Inhibited by DBeQ

Yahui Zhang, Wanjie Li, Mi Chu, Hengye Chen, Haoyuan Yu, Chaoguang Fang, Ningze Sun, Qiming Wang, Tian Luo, Kaiju Luo, Xueping She, Mengqian Zhang, Dong Yang

Mycopathologia. 2016 Jun;181(5-6):329-39.

PMID: 26700222

Abstract:

Candida albicans is an opportunistic human pathogen, and its pathogenicity is associated with hyphal formation. Previous studies have shown that at neutral-to-alkaline pH, hyphal growth is dependent on the Rim101 pathway whose activation requires Snf7, a member of the ESCRT system. In this work, we described the purification and characterization of the C. albicans Vps4, an AAA ATPase required for recycling of the ESCRTs. Its role on hyphal growth has been investigated. Our data suggest deletion of Vps4 decreases overall hyphal growth at pH 7 and increases the growth of multiple hyphae induced by serum, which indicates that the ESCRTs may make a Rim101-independent contribution to hyphal growth. Furthermore, DBeQ, an inhibitor of the AAA ATPase p97, was shown to inhibit the ATPase activity of Vps4 with an IC50 of about 11.5 μM. To a less degree, it also inhibits hyphal growth. Our work may provide a new strategy to control C. albicans infection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4242996	DBeQ			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: