CAS 9051-97-2 β-1,3-Glucan from Euglena gracilis - Analytical Products / Alfa Chemistry

CAT	AP9051972
CAS	9051-97-2
Structure
MDL Number	MFCD00466918

Size

1G, 5G

Bioproducts From Euglena gracilis: Synthesis and Applications

Alexander Gissibl, Angela Sun, Andrew Care, Helena Nevalainen, Anwar Sunna

Front Bioeng Biotechnol. 2019 May 15;7:108.

PMID: 31157220

Identification of Euglena gracilis β-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149

Sakonwan Kuhaudomlarp, Nicola J Patron, Bernard Henrissat, Martin Rejzek, Gerhard Saalbach, Robert A Field

J Biol Chem. 2018 Feb 23;293(8):2865-2876.

PMID: 29317507

Improvement of Euglena Gracilis Paramylon Production Through a Cocultivation Strategy With the Indole-3-Acetic Acid-Producing Bacterium Vibrio Natriegens

Jee Young Kim, Jeong-Joo Oh, Min Seo Jeon, Gyu-Hyeok Kim, Yoon-E Choi

Appl Environ Microbiol. 2019 Sep 17;85(19):e01548-19.

PMID: 31324633

Paramylon, a Potent Immunomodulator from WZSL Mutant of Euglena gracilis

Laura Barsanti, Paolo Gualtieri

Molecules. 2019 Aug 27;24(17):3114.

PMID: 31461965

β-1,3 Glucan Derived From Euglena Gracilis and Algamune™ Enhances Innate Immune Responses of Red Drum (Sciaenops Ocellatus L.)

Fernando Y Yamamoto, Fei Yin, Waldemar Rossi Jr, Michael Hume, Delbert M Gatlin 3rd

Fish Shellfish Immunol. 2018 Jun;77:273-279.

PMID: 29625243

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Case Study

β-1,3-Glucans from Euglena gracilis for Ulcerative Colitis Prevention via Gut Barrier Enhancement and Microbiota Modulation

Guo, Bingbing, et al. "β-1, 3-glucans from Euglena Gracilis protects against ulcerative colitis via modulating the gut barrier, T-cell immunity and gut microbiota." International Journal of Biological Macromolecules (2025): 141288.

β-1,3-Glucans from Euglena gracilis (EGP) have emerged as a potent bioactive compound for mitigating ulcerative colitis (UC) by targeting gut barrier integrity, immune regulation, and microbiota composition. Using a dextran sulfate sodium (DSS)-induced colitis mouse model, EGP demonstrated significant protective effects, reducing body weight loss, improving colon length, and lowering disease activity index (DAI).
EGP's immunomodulatory properties were evident in its ability to suppress pro-inflammatory cytokines TNF-α and IL-6 while restoring the Th17/Treg balance, a crucial factor in immune homeostasis. Additionally, EGP upregulated the expression of the tight junction protein ZO-1, reinforcing gut barrier function. Gut microbiota analysis revealed that EGP enriched beneficial bacterial populations, particularly Bacteroidota, while promoting tryptophan metabolism to generate anti-inflammatory metabolites.
In vitro fermentation studies using fecal samples from UC patients confirmed EGP's prebiotic potential, increasing the abundance of Clostridiaceae and Lactobacillaceae. These findings establish β-1,3-glucans from Euglena gracilis as a promising functional ingredient for UC prevention, supporting the development of next-generation nutraceuticals and prebiotic therapies aimed at gut health and immune modulation.

β-1,3-Glucan (BG) for Nanophytosome-Based Drug Delivery: Enhanced Bioavailability via Phosphatidylcholine Encapsulation

Palol, Varsha Virendra, et al. MethodsX 11 (2023): 102480.

β-1,3-Glucan (BG) from Euglena gracilis is a bioactive polysaccharide with immunomodulatory properties, but its high molecular weight often limits absorption and bioavailability. To overcome this, a novel lipid-based nanophytosome (NP) delivery system was developed by encapsulating BG with phosphatidylcholine (PC) via a solvent evaporation method.
The synthesis process involved dissolving BG in dimethyl sulfoxide (DMSO) under controlled heating and combining it with PC under magnetic stirring, followed by ultrasonication and rotary evaporation. Dynamic Light Scattering (DLS) measurements confirmed nanophytosome sizes of 134.62 nm (BG-PC) and 158.38 nm (EG-PC), ensuring optimal cellular uptake. Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD) analysis demonstrated enhanced binding efficiency and the amorphous nature of the encapsulated BG. Morphological characterization by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) revealed spherical vesicular structures, further supporting efficient encapsulation.
The BG-PC formulation achieved an encapsulation efficiency of 82 ± 1.62%, significantly improving its stability and bioavailability for pharmaceutical applications. This study establishes β-1,3-glucan nanophytosomes as a promising drug delivery platform, paving the way for further exploration in medical and nutraceutical formulations. Future research should focus on evaluating their long-term stability and therapeutic efficacy for clinical use.

β-1,3-Glucan from Euglena gracilis as an Immunostimulant in Swimming Crab Hemocytes: Enhancing Innate Immunity and Antiparasitic Activity

Perveen, Summia, et al. Fish & Shellfish Immunology 114 (2021): 28-35.

β-1,3-Glucan from Euglena gracilis has demonstrated significant immunomodulatory effects, making it a promising immunostimulant in aquaculture. In this study, the immunostimulatory potential of β-1,3-glucan was assessed in Portunus trituberculatus hemocytes via in vitro stimulation. The results revealed that β-1,3-glucan significantly enhanced cellular enzyme activities, including phenoloxidase (PO), lysozyme, acid phosphatase (ACP), and superoxide anion generation (O2-) at both intracellular and extracellular levels. Additionally, superoxide dismutase (SOD) activity was significantly increased in β-glucan-stimulated hemocytes.
Gene expression analysis showed that β-1,3-glucan upregulated immune-related genes such as proPO, TLR-2, Lysozyme, and Crustin-1. Furthermore, a dose-dependent antiparasitic activity was observed against Mesanophrys sp., with significant reductions in parasite growth in the β-glucan-treated hemocytes compared to the control.
This study highlights the potential of β-1,3-glucan from Euglena gracilis as an effective immunostimulant for enhancing innate immunity and providing antiparasitic protection in swimming crab aquaculture. The results support its use in boosting the immune response and improving disease resistance in crustacean farming. Further research could optimize its application in aquaculture practices.