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Two-dimensional Liquid Chromatography Analysis of All-Trans-, 9-cis- And 13-cis- Astaxanthin in Raw Extracts From Phaffia Rhodozyma

Two-dimensional Liquid Chromatography Analysis of All-Trans-, 9-cis- And 13-cis- Astaxanthin in Raw Extracts From Phaffia Rhodozyma

Xiping Du, Chun Wang, Ling Wu, Zhipeng Li, Faizan Ahmed Sadiq, Zedong Jiang, Feng Chen, Hui Ni, Qingbiao Li

J Sep Sci. 2020 Jun 7.

PMID: 32506706

Abstract:

An effective two-dimensional liquid chromatography method has been established for the analysis of all-trans-astaxanthin and its geometric isomers from Phaffia rhodozyma employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10-port valve using the photo-diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32-1.14% and 98.21-106.13%, respectively. By using two-dimensional liquid chromatography, it was found that day light, ultrasonic treatment and heat treatment have significant influence on the content of all-trans-astaxanthin in the extract from P. rhodozyma, due to the transformation of all-trans-astaxanthin to cis-astaxanthin. The day light and ultrasonic treatments more likely transform all-trans-astaxanthin to 9-cis-astaxanthin, and the thermal treatment transforms all-trans-astaxanthin to 13-cis-astaxanthin. These results indicate the two-dimensional LC method can facilitate monitoring astaxanthin isomerization in the raw extract from P. rhodozyma. In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers. This article is protected by copyright. All rights reserved.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP113085044	9-cis-Astaxanthin	113085-04-4	Price
AP113085055	13-cis-Astaxanthin	113085-05-5	Price
AP472617-A	all-trans-Astaxanthin	472-61-7	Price

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